Melatonin Improves The Developmental Competence of Goat Oocytes

Authors

  • Hoda Rajabi Department of Transgenic Animal Science, Stem Cell Technology Research Center, Tehran, Iran
  • Kazem Parivar Department of Biology, Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran
  • Saghar Saeedabadi Department of Biology, Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran
Abstract:

Objective DNA methylation is one the epigenetic mechanisms, which is critically involved in gene expression. This phenomenon is mediated by DNA methyl-transferases and is affected by environmental stress, including in vitro maturation (IVM) of oocytes. Melatonin, as an antioxidant, may theoretically be involved in epigenetic regulation via reductions of reactive oxygen species. This study was performed to investigate DNA methylation and the possibility of goat oocyte development after treatment with different concentrations of melatonin. MaterialsAndMethods This experimental study was performed to investigate DNA methylation and the possibility of goat oocyte development after treatment with different concentrations of melatonin. For this purpose, oocytes with granulated cytoplasm were selected and co-cultured with at least two layers of cumulus cells in maturation medium with 10-6 M, 10-9 M, 10-12 M and 0-M (as control group) of melatonin. Nucleus status, glutathione content and developmental competence of the oocytes in each experimental group were assessed. Also, expression of genes associated with DNA methylation, including DNA methyltransferase 1 (DNMT1), DNA methyltransferase 3b (DNMT3b) and DNA methyltransferase 3a (DNMT3a) was evaluated by quantitative real time-polymerase chain reaction (RT-PCR). Results According to our findings, the percentage of oocytes that reached the M-II stage significantly increased in the 10-12 M group (P

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Journal title

volume 12  issue 2

pages  157- 163

publication date 2018-03-01

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